DMSO master liquid, next add L PEG300 mix and clarify, next add L.You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent.Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.The Selleck molarity calculator is based on the following equation.
The Selleck dilution calculator is based on the following equation. In this example, I put the volumes in microliters, because thats how our micropipets work. Bio 6B Calendar Lecture lab Bio 6B Syllabus Winter 2019 Go to Bio 6A Bio 6AB intro page Special Projects Summer Research Finding a summer research program. Almost all of them are either conversions or dilutions, so two basic kinds of equations will solve all your problems. For the labs in this course, there are only a few kinds of units, and the prefixes that go with them. In this lab, well usually express concentrations in M or mM. Well use these units for DNA and proteins, which are variable in size. For example, if you start with an unknown concentration of a bacterial culture, you could call it X. If you do a 10-fold dilution, youll have a concentration of 0.1X. You dont need to remember that a nanogram is 10 -9 grams; just remember that there are 1000 ng in 1 g and 1000 g in 1 mg and 1000 mg in 1 g. Most of the lab experiments are done with a Pipetman, which has a practical precision of three significant figures. If youre using a P-2 to pipet 1 l, youd set it at 1.00 l, so 1.00 l would be the correct answer for a quiz. If the answer is 0.10 l, you dont need to write 0.100 l, because you dont set the pipet that way. An answer like 0.00113045 ml would definitely be wrong; it might be mathematically correct, but its unrealistic in its precision and impractical in terms of how youd pipet it. The unwanted units should cancel out, leaving you with the units you want. Many students want to reach for the calculator first, without writing out the equation; often, this leads to the wrong answer. You want to make 1 ml of DNA solution with a final concentration of 1 mgml. In this example, the units are the same on both sides of the equation, but that wont always be the case. In the bio lab, you will often have other concentration units, so its best to think of the equation as C 1 V 1 C 2 V 2 instead of M 1 V 1 M 2 V 2. The concentration of the bacteria is unknown, so youll have to call it 1x. You need to dilute this culture a lot, to get 1 ml of 10 -6 x solution. How do you do the dilution You could try the dilution this way. The problem is that you cant set your pipetman to 10 -6 ml, or 0.001 l (1 nl). Alternatively, you could look at it like this. We dont have a sterile culture tube that holds 10 6 ml, or 1000 liters. C1V1 C2V2 Calculator Series Of DilutionsThe only practical way to do large dilutions is to do a series of dilutions: make a 10 -2 x dilution, use that to make a 10 -4 x dilution, then use the 10 -4 x dilution to make the 10 -6 x.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |